BEGIN:VCALENDAR VERSION:2.0 PRODID:IEEE vTools.Events//EN CALSCALE:GREGORIAN BEGIN:VTIMEZONE TZID:America/Denver BEGIN:DAYLIGHT DTSTART:20260308T030000 TZOFFSETFROM:-0700 TZOFFSETTO:-0600 RRULE:FREQ=YEARLY;BYDAY=2SU;BYMONTH=3 TZNAME:MDT END:DAYLIGHT BEGIN:STANDARD DTSTART:20261101T010000 TZOFFSETFROM:-0600 TZOFFSETTO:-0700 RRULE:FREQ=YEARLY;BYDAY=1SU;BYMONTH=11 TZNAME:MST END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTAMP:20260525T194754Z UID:B556D42B-B349-47BE-9466-F9B6A39C25D3 DTSTART;TZID=America/Denver:20260529T110000 DTEND;TZID=America/Denver:20260529T120000 DESCRIPTION:Methane and methanol are inexpensive and sustainable carbon fee dstocks that can be used by methanotrophic bacteria as sole sources of car bon and energy and can be converted into value-added products. On the othe r hand\, isoprenoids are valuable compounds that can be used for high perf ormance fuels\, flavoring agents and pharmaceuticals or for precursors to such products\; however\, methanotrophs do not readily produce isoprenoids . Methylomicrobium album BG8 is a methanotroph with high methanol toleranc e and has been grown to high density in fed-batch operation. In this work\ , M. album BG8 is being modified to convert methane and methanol into isop renoids. M. album BG8 contains the doxp pathway\, which produces isopenten yl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)\, two isoprenoi d precursors\, but it lacks enzymes which are required to transform these compounds into isoprenoids. In addition\, works in other bacterial systems suggest low expression of the native doxp pathway leading to a potential bottleneck in the generation of isoprenoids. In this work\, conjugation wa s used to modify M. album BG8 with plasmids for high and low expression le vels of the genes involved in isoprenoid production. A library of constitu tive and inducible promoters was developed using green fluorescent protein (GFP) and nanoluc as markers. Co-transformation of high and low expressio n vectors are being performed to improve the flux through the doxp pathway and\, eventually\, isoprenoid synthesis. This work provides a basis for t he production of isoprenoids from methane and methanol by bacteria\, an ap proach with great economic and environmental potential.\n\nCo-sponsored by : Resilience and Clean Energy Systems (RCES)\n\nSpeaker(s): Shibashis Das\ n\nVirtual: https://events.vtools.ieee.org/m/561182 LOCATION:Virtual: https://events.vtools.ieee.org/m/561182 ORGANIZER:bli4@ualberta.ca SEQUENCE:15 SUMMARY:Engineering a methanol-tolerant methanotroph for conversion of meth ane and methanol to isoprenoids URL;VALUE=URI:https://events.vtools.ieee.org/m/561182 X-ALT-DESC:Description: <br /><p class="MsoNormal"><span lang="EN-US">Metha ne and methanol are inexpensive and sustainable carbon feedstocks that can be used by methanotrophic bacteria as sole sources of carbon and energy a nd can be converted into value-added products. On the other hand\, isopren oids are valuable compounds that can be used for high performance fuels\, flavoring agents and pharmaceuticals or for precursors to such products\; however\, methanotrophs do not readily produce isoprenoids. <em>Methylomic robium album</em> BG8 is a methanotroph with high methanol tolerance and h as been grown to high density in fed-batch operation. In this work\, <em>M . album</em> BG8 is being modified to convert methane and methanol into is oprenoids. <em>M. album</em> BG8 contains the doxp pathway\, which produce s isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)\, tw o isoprenoid precursors\, but it lacks enzymes which are required to trans form these compounds into isoprenoids. In addition\, works in other bacter ial systems suggest low expression of the native doxp pathway leading to a potential bottleneck in the generation of isoprenoids. In this work\, con jugation was used to modify <em>M. album </em>BG8 with plasmids for high a nd low expression levels of the genes involved in isoprenoid production. A library of constitutive and inducible promoters was developed using green fluorescent protein (GFP) and nanoluc as markers. Co-transformation of hi gh and low expression vectors are being performed to improve the flux thro ugh the doxp pathway and\, eventually\, isoprenoid synthesis. This work pr ovides a basis for the production of isoprenoids from methane and methanol by bacteria\, an approach with great economic and environmental potential .</span></p> END:VEVENT END:VCALENDAR