Advances in broadband coherent Raman microscopy instrumentation: a coherent Raman platform for biomedical imaging
In the realm of label-free imaging techniques, coherent Raman imaging (CRI) emerges as a powerful tool, offering sub-cellular spatial resolution, molecular-specific contrast, and addressing the unmet need in life sciences for label-free chemically specific imaging by detecting the intrinsic vibrational fingerprints of cells and tissues [1].
Multiplex stimulated Raman scattering (SRS) microscopy [2], combining single-shot detection of broad vibrational spectra and high spectral resolution, fully exploits the innovative potential of CRI tools. State of the art implementations of multiplex SRS systems are based on custom and complex solutions, rendering them completely inaccessible to non-specialists in the field [2,3].
Here, we present a fully engineered Broadband Coherent Raman Platform – CORAL designed to achieve state-of-the-art performance in multiplex SRS with unprecedented ease of use and long-term reproducibility. CORAL comprises an all-fiber dual-wavelength self-synchronized laser and a detection unit based on a compact multichannel lock-in amplifier, ensuring shot-noise-limited SRS performance over the entire CH spectrum (2800-3100 cm⁻¹), parallelizing detection across 38 spectral channels in 2 μs. Additionally, the system is equipped with an epi-detection module for TPEF and SHG signals.
Moreover, CORAL combines a broadband label-free approach for chemometric analysis of biological specimens with artificial intelligence tools, enabling users to unleash the full power of hyperspectral data. Such a system finds broad application in biomedical sectors where traditional exogenous labeling is a limiting factor, such as in live cell imaging, metabolomics, and histopathology.
[1] Zhang, C. & Cheng, J.-X. Perspective: Coherent Raman scattering microscopy, the future is bright. APL Photonics vol. 3 090901 (2018).
[2] Fu, D. et al. Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy. Journal of the American Chemical Society vol. 134 3623–3626 (2012).
[3] De la Cadena, A. et al. Broadband stimulated Raman imaging based on multi-channel lock-in detection for spectral histopathology. APL Photonics vol. 7 076104 (2022).
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Dr Matteo Negro of Chief Executive Officer at Cambridge Raman Imaging Ltd
Advances in broadband coherent Raman microscopy instrumentation: a coherent Raman platform for biomedical imaging
In the realm of label-free imaging techniques, coherent Raman imaging (CRI) emerges as a powerful tool, offering sub-cellular spatial resolution, molecular-specific contrast, and addressing the unmet need in life sciences for label-free chemically specific imaging by detecting the intrinsic vibrational fingerprints of cells and tissues [1].
Multiplex stimulated Raman scattering (SRS) microscopy [2], combining single-shot detection of broad vibrational spectra and high spectral resolution, fully exploits the innovative potential of CRI tools. State of the art implementations of multiplex SRS systems are based on custom and complex solutions, rendering them completely inaccessible to non-specialists in the field [2,3].
Here, we present a fully engineered Broadband Coherent Raman Platform – CORAL designed to achieve state-of-the-art performance in multiplex SRS with unprecedented ease of use and long-term reproducibility. CORAL comprises an all-fiber dual-wavelength self-synchronized laser and a detection unit based on a compact multichannel lock-in amplifier, ensuring shot-noise-limited SRS performance over the entire CH spectrum (2800-3100 cm⁻¹), parallelizing detection across 38 spectral channels in 2 μs. Additionally, the system is equipped with an epi-detection module for TPEF and SHG signals.
Moreover, CORAL combines a broadband label-free approach for chemometric analysis of biological specimens with artificial intelligence tools, enabling users to unleash the full power of hyperspectral data. Such a system finds broad application in biomedical sectors where traditional exogenous labeling is a limiting factor, such as in live cell imaging, metabolomics, and histopathology.
[1] Zhang, C. & Cheng, J.-X. Perspective: Coherent Raman scattering microscopy, the future is bright. APL Photonics vol. 3 090901 (2018).
[2] Fu, D. et al. Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy. Journal of the American Chemical Society vol. 134 3623–3626 (2012).
[3] De la Cadena, A. et al. Broadband stimulated Raman imaging based on multi-channel lock-in detection for spectral histopathology. APL Photonics vol. 7 076104 (2022).
Biography:
Matteo Negro is Chief Executive Officer at Cambridge Raman Imaging Ltd, where he leads the company after having built a technical team with expert knowledge in the critical technology areas. Originally as a CTO he has led product design and development. Dr Negro’s previous role was as Laser R&D manager for Lumenis, a global leader in medical lasers. He is expert in laser development whilst meeting the demanding requirements of ISO 13485 for medical devices, having excellent experience of R&D, including managing the development of laser systems which gained Class IIb medical approval. Dr Negro has a PhD in Physics from Politecnico di Milano on Ultrafast laser sources and spectroscopy and has previously worked as a Staff Researcher at the Institute of Photonics and Nanotechnologies of the Italian National Research Council (CNR-IFN).
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